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「电子书」SARS- and Other Coronaviruses: Laboratory Protocols SARS-和其他冠状病毒:实验室规程 PDF

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2003年,"冠状病毒 "这个词在全球范围内传播,比引发恐慌的病毒传播得更远一些。在《SARS-和其他冠状病毒。实验室协议》中,专家研究人员通过详细的实验室协议来研究这些毁灭性的病毒。各章涉及的主题包括:用核酸和抗体方法检测和发现冠状病毒,病毒分离、传播和滴定,病毒纯化,电子冷冻显微镜的结构分析,病毒蛋白的表达和结晶,培养针对病毒蛋白的抗体,冠状病毒基因组的操作,以及如何研究细胞表面冠状病毒受体的描述。本书采用非常成功的Methods in Molecular Biology™系列格式编写,每一章都包含简短的介绍、逐步的方法、必要的材料清单,以及 "笔记 "部分,该部分分享了有关故障排除和避免已知陷阱的提示。


《SARS-和其他冠状病毒。实验室规程》是所有病毒学家,特别是从事冠状病毒工作的人员的理想指南。


In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.


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